List of Experiment In Bio Tech. Department

Experiment	Name of the Equiment required
Semester V
Recombinant DNA Technology lab	Gel electrophoresis system
1. Target selection 2. Strategy for cloning 3. Primer design 4. Isolation of genomic DNA	(horizontal and vertical), high-speed Centrifuge, PCR (Polymerase chain reaction), Western blotting apparatus, Gel plate rocker.
5. Gene amplification by PCR
6. Ligation of desired gene sequence
7. Transformation
8. Verification of cloned DNA
9. Induction of expression
10. Verification of protein expression
*Fermentation and Downstream Processing*
Lab
1. Study of factors affecting bioprocesses in
submerged fermenters (pH, O2, Temperature,
Foam, Ingredients)
2. Purify a bacterial protein
a. Cell lysis by different methods.
b. Cell debris separation by different
methods.
c. Column purification
i. Separation by Molecular
weight.
ii. By charge.
iii. By metal affinity.
iv. By Receptor-Ligand affinity.
d. Dialysis
e. Ultrafiltration
f. Crystallization	*
g. Lyophilization
3. Purification of O-PS
a) Cell lysis
b) Harvesting of cells
c) Purification of O-PS antigens

List of Experiements Bio Technology Department

Diagnostic Techniques & Biostatistical Analysis Lab.

1. Enzyme Immunoassay in Plates

a. Conjugation of antibody with
enzyme.

b. OD vs. Antigen cone, plot and its
analysis.

2. Point of Care -Membrane based assays

a. Dipstick based assays

b. Immunofiltration

c. Lateral flow assays

3. Immunoblots

4. DNA primer probe selection

a. Design probes for diseases

b. Use commercially available probes
to amplify gene fragment in clinical
samples.

c. Detection of amplified product

1. Gel electrophoresis

2. Dot Blots

3. Hybrid capture assays

5. Genotyping HLA

6. DNA Fingerprinting

7. Biostatistical analysis of scientific data

Elisa Plate reader, Gel electrophoresis system (horizontal and vertical), high-speed Centrifuge, Western blotting apparatus, Gel plate rocker, PCR

Semester VI

Cell and Tissue Culture Lab A) Animal Cell Culture

1) Primary cell culture from 12-14 days mouse
embryos.

2) Culture of fibroblast and epithelial cells.

3) Visualization of proteins by
immunofluorescence.

4) Induction of interferon in cell culture.

5) Culture of human lymphocytes

• PHA stimulation-estimation by
amount of DNA.

• Karyotyping.

Carbon dioxide incubator, autoclave, Laminar flow, Phase contrast microscope,

List of Experiements Bio Technology Department

B) Plant Tissue Culture
1) Plant cell culture from different types of
explants.
2) Isolation of DNA/RNA from cultured cells	■■
and compare with seeds.
3) Callus development for somatic
embryogenesis.
4) Agrobacterium mediated transformation.
Healthcare Biotechnology Lab.
1. Normal microflora of mouth and determination
of its susceptibility to dental caries.	Autoclave, Laminar flow, UV-
2. Preliminary identification of enteric bacteria	Spectrophotometer, Water-bath,
by TSI agar.	Oven
3. Estimation of ketone bodies in the urine of
normal persons.
4. Fasting and post-prandial determination of
blood glucose.
5. Lipid profile (Total cholesterol, TG, VLDL,
LDL and HDL) of human blood samples.
6. Determination of Rh factor.
7. VDRL test for syphilis.
8. WIDAL test for Salmonella/Typhoid.
9. Pregnancy test.
10. Cultural sensitivity of pathogens against
various antibiotics.
Microbial, Food and Environmental
Biotechnology Lab.
A. Microbial Biotechnology: 1. Production of organic acids (Citric and lactic) by microorganisms. 2. Production of antibiotics by	Autoclave, Incubator, Laminar flow Colony counter, Water-bath, BOD incubator, conductivity meter,
microorganisms.	*
3. Production of industrially important
enzymes (protease, amylase, cellulose,
xylase or lipase) by microorganisms.
4. Demonstration of bacterial lysis by
bacteriophages.
B. Food Biotechnology:
1. Microbiological analysis (bacterial
count) of food products.

List of Experiements Bio Technology Department

2. Isolation of bacteriocin producing
microorganisms from fermented foods
and determination of the antimicrobial
spectrum of bacteriocin producing
isolates.

3. Cultivation of white button mushroom
(Agaricus biosporus).

4. Determination of food toxins.
C. Environmental Biotechnology:

1. Qualitative analysis of water/waste water:

■ a. Bacterial analysis

b. Determination of hardness, alkalinity,
conductivity, chlorides, temperature
and pH.

c. Determination of soluble phosphates.

d. Determination of BOD, COD and DO
contents.

2. Biobleaching/ Decolourization of industrial
effluents/industrially important dyes by
microbes.

3. Microbial degradation of organic wastes eg:
Lignocelluloses/ pesticides/ hydrocarbons.

4. Vermicomposting.

Semester VII

BIOINFORMA TICS LABORA TOR Y

1. Computer basics

2. Searching biological database for relevant

3. Data mining techniques in Bioinformatics.

4. Searching, retrieval and similarity analysis of
biological database.

5. Sequence retrieval from nucleic acid and protein
database.

6 Restriction mapping

7. Sequence (FASTA & BLAST) searches.

8. Pair wise comparison of sequences.

9. Evolutionary studies/ Phylogenic analysis.

10. Identification of genes in genomes.

11. Protein databank retrieval and visualization.

12. Superposition of structures.

13. Secondary structure prediction of proteins.

14. Pattern searching in nucleic acids.

15. Validation of 3D structures.

Computers, Colour printer, Softwares,

•

B.TECH IIIRD SEMESTER

ANALOG COMMUNICATION LAB

(ECE-207E)

LIST OF EXPERIMENTS: -

1. Study of AM Modulation/Demodulation

2. Study of FM Modulation/Demodulation.

3. Study of Diode detector and AGC.

4. To study Sampling theorem.

5. Sensitivity of a superhet Receiver.

6. Selectivity of a superhet Receiver.

7. Fidelity of a superhet Receiver.

8. Study of Pulse Amplitude Modulation/Demodulation.

9. Study of Pulse Width Modulation/Demodulation.

10. Study of Pulse Position Modulation/Demodulation.

NOTE:

At least seven experiments are to be performed from above list and the concerned institution as per the scope of the syllabus can set remaining three.

EE-324-E CONTROL SYSTEM LAB

LIST OF EXPERIMENTS :

1. To study A.C. servo motor and to plot its torque speed characteristics.

2. To study D.C. servo motor and to plot its torque speed characteristics.

3. To study the magnetic amplifier and to plot its load current v/s control current characteristics for :

(a) series connected mode

(b) parallel connected mode.

4. To plot the load current v/s control current characteristics for self exited mode of the magnetic amplifier.

5. To study the synchro & to:

(a) Use the synchro pair (synchro transmitter & control transformer) as an error detector.

(b)Plot stator voltage v/s rotor angle for synchro transmitter i.e. to use the synchro transmitter as position transducer.

6. To use the synchro pair (synchro transmitter & synchro motor) as a torque trans mitter.

7. (a) To demonstrate simple motor driven closed loop position control system.

(b) To study and demonstrate simple closed loop speed control system.

8. To study the lead, lag, lead-lag compensators and to draw their magnitude and phase plots .

9. To study a stepper motor & to execute microprocessor or computer-based control of the same by changing number of steps, direction of rotation & speed.

10. To implement a PID controller for level control of a pilot plant.

11. To implement a PID controller for temperature control of a pilot plant.

12. To study the MATLAB package for simulation of control system design.

NOTE : At least ten experiments have to be performed in the semester, at least seven experiments should be performed from above list. Remaining three experiments may either be performed from the above list or designed & set by the concerned institution as per the scope of the syllabus of EE-304-C.

B.TECH IVTH SEMESTER

DIGITAL ELECTRONICS LAB

(ECE-210E)

LIST OF EXPERIMENTS:

1. Familiarization with Digital Trainer Kit and associated equipment.

2. Study of TTL gates AND, OR, NOT, NAND, NOR, EX-OR, EX-NOR.

3. Design and realize a given function using K-Maps and verify its performance.

4. To verify the operation of Multiplexer and Demultiplexer.

5. To verify the operation of Comparator.

6. To verify the truth table of S-R, J-K, T, D Flip-flops.

7. To verify the operation of Bi-directional shift register.

8. To design and verify the operation of 3-bit asynchronous counter.

9. To design and verify the operation of asynchronous Up/down counter using J-K FFs.

10. To design and verify the operation of asynchronous Decade counter.

11. Study of TTL logic family characteristics.

12. Study of Encoder and Decoder.

13. Study of BCD to 7 segment Decoder.

NOTE:

Eight questions are to be set in all by the examiner taking two questions from each unit. Students will be required to attempt five questions in all.

DIGITAL SYSTEM DESIGN LAB

LIST OF EXPERIMENTS:

1. Design all gates using VHDL.

2. Write VHDL programs for the following circuits, check the wave forms and the hardware generated

a. half adder

b. full adder

3. Write VHDL programs for the following circuits, check the wave forms and the hardware generated

a. multiplexer

b. demultiplexer

4. Write VHDL programs for the following circuits, check the wave forms and the hardware generated

a. decoder

b. encoder

5. Write a VHDL program for a comparator and check the wave forms and the hardware generated

6 Write a VHDL program for a code converter and check the wave forms and the hardware generated

7. Write a VHDL program for a FLIP-FLOP and check the wave forms and the hardware generated

8. Write a VHDL program for a counter and check the wave forms and the hardware generated

9. Write VHDL programs for the following circuits, check the wave forms and the hardware generated

a. register

b. shift register

10. Implement any three (given above) on FPGA/CPLD kit

Note : Ten experiments are to be performed out of which at least seven experiments should be performed from above list. Remaining three experiments may either be performed from the above list or designed & set by the concerned institution as per the scope of the syllabus.

ELE – 203E ELECTRICAL MACHINES LAB

LIST OF EXPERIMENTS

1. To perform open and short circuit tests on 1-phase transformer and to calculate efficiency.

2. To perform Sumpner’s back to back test on-phase transformer.

3. Parallel operation of two 1-phase transformers.

4. Study of construction of a DC machine.

5. To plot magnetizing of a DC SE Generator and find its critical resistance & critical speed.

6. Speed Control of a DC motor by armature control & field control methods.

7. Open circuit & Block test of 1-phase induction motor.

8. Light running & block rotor test of 3-phase I.M. with starting.

9. To plot V curve of a synchronous motor.

10. To study scott connection of transformer.

11. To study starting running & reversal of direction of 3-phase I.M.

12. To perform load test on a 3-phase I.M. D.C. generator set & to determine the efficiency of I.M.

NOTE : Ten experiments are to be performed, out of which at least seven experiments should be performed from above list. Remaining three experiments may either be performed from the above list or designed & set by the concerned institution as per the scope of the syllabus.

B.TECH IVTH SEMESTER

ELECTRONICS MEASUREMENTS LAB

(ECE-208E)

LIST OF EXPERIMENTS

To measure the unknown Inductance in terms of capacitance and resistance by using Maxwell’s Inductance bridge.
To measure unknown Inductance using Hay’s bridge.
To measure unknown capacitance of small capacitors by using Schering’s bridge.
To measure 3-phase power with 2-Wattmeter method for balanced and unbalanced bridge.
To measure unknown capacitance using De-Sauty’s bridge.
To measure unknown frequency using Wein’s frequency bridge.
To measure unknown low resistance by Kelvin’s Double bridge.
To test the soil resistance using Meggar (Ohm meteTo calibrate Energy meter using standard Energy meter.
To plot the B-H curve of different magnetic materials.
To calibrate the Voltmeter using Crompton Potentiometer.
To convert the Voltmeter into Ammeter using Potentiometer.
Insulation testing of cables using Digital Insulation Tester.

NOTE:

Eight questions are to be set in all by the examiner taking two questions from each unit. Students will be required to attempt five questions in all.

MICROWAVE LAB

L T P CLASS WORK : 25

0 0 2 EXAM : 25

TOTAL : 50

DURATION OF EXAM : 3 HRS

LIST OF EXPERIMENTS :

1. Study of wave guide components.

2. To study the characteristics of reflex Klystron and determine its timing range.

3. To measure frequency of microwave source and demonstrate relationship among guide dimensions, free space wave length and guide wavelength.

4. To measure VSWR of unknown load and determine its impedance using a smith chart.